Recent Innovations in High-Performance Liquid Chromatography (HPLC): Method Development and Validation Strategies

Abstract

High-Performance Liquid Chromatography (HPLC) has established itself as a cornerstone in analytical chemistry, playing a pivotal role in the separation, identification, and quantification of compounds in complex mixtures. Over the years, significant advancements in HPLC method development and validation have enhanced its precision, accuracy, and application scope across pharmaceutical, environmental, and food industries. This review delves into recent innovations in HPLC, emphasizing robust method development strategies that ensure reproducibility and reliability. Key aspects, such as the selection of stationary and mobile phases, optimization of chromatographic parameters, and advanced gradient programming, are explored in detail. Additionally, this review highlights contemporary validation techniques, including system suitability testing, linearity assessment, and specificity evaluation, which are critical to meeting regulatory compliance. Recent technological advancements, such as the integration of chemometric tools, high-resolution detectors, and environmentally friendly solvents, are also discussed, showcasing their impact on method efficiency and sustainability. The article aims to provide a comprehensive understanding of HPLC method development and validation for researchers and industry professionals, bridging the gap between traditional practices and modern innovations. By summarizing challenges and proposing future directions, this review underscores the evolving nature of HPLC as a versatile analytical tool in various scientific domains.